Research Thrust 4
Homeostasis of Cultured Mammalian Cells in a Nanofiber Environment
Objectives: To develop nanofiber (NF) based scaffolds to analyze the interaction of cultured mammalian cells and explore novel methods for monitoring cellular viability and homeostasis in a NF environment.
Faculty: UTRGV: Robert Gilkerson (lead), Karen Lozano, and Waseem Haider. UMN: Theresa Reineke.
Fig. 17 Mitochondria balance fission and fusion. Confocal imaging (anti-TOM20 immunolabeling, green) of mitochondria undergoing fusion (left) and fission (right) in 143B osteosarcoma cells. n=3 expts.
Fig. 16 Confocal microscopy of 3T3 fibroblasts seeded onto NFs. Cells were visualized for mitochondria (MitoTracker, red) and nuclei (DAPI, blue), while NF filaments appear blue.