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Research Description Thrust 4

Partnership for Research and Education in Materials Science College of Engineering and Computer Science

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Research Thrust 4

Homeostasis of Cultured Mammalian Cells in a Nanofiber Environment

Objectives: To develop nanofiber (NF) based scaffolds to analyze the interaction of cultured mammalian cells and explore novel methods for monitoring cellular viability and homeostasis in a NF environment.

Faculty: UTRGV: Robert Gilkerson (lead), Karen Lozano, and Waseem Haider. UMN: Theresa Reineke.

mitochondria undergoing fusion (left) and fission (right)

Fig. 17 Mitochondria balance fission and fusion. Confocal imaging (anti-TOM20 immunolabeling, green) of mitochondria undergoing fusion (left) and fission (right) in 143B osteosarcoma cells. n=3 expts.

Confocal microscopy of 3T3 fibroblasts seeded onto NFs.

Fig. 16 Confocal microscopy of 3T3 fibroblasts seeded onto NFs. Cells were visualized for mitochondria (MitoTracker, red) and nuclei (DAPI, blue), while NF filaments appear blue.

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